Use of cell separation and short-term culture techniques to study erythroid cell development

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Use of cell separation and short-term culture techniques to study erythroid cell development.

Cell populations highly enriched for the different stages of erythroid cell maturation were obtained by three sequential operations: harvesting of erythroid cells after induction of erythroid hyperplasia in the spleens of mice, elimination of the more mature erythrocytes by immunologic techniques, and separation of the residual nucleated erythroid cells as a function of size by the velocity sed...

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Cell Culture and Induction of Erythroid Differentiation

Physicochemical and immunologic properties of 6-aminolevulinate (ALA) dehydratase in human K562 erythroleukemia cells were examined. ALA dehydratase activity was found to increase in K562 cells after treatment with butyric acid or selenium oxide. Enzyme activity in untreated K562 cells was comparable to that in normal adult erythrocytes but was increased threeto six-fold in K562 cells treated w...

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ژورنال

عنوان ژورنال: Blood

سال: 1975

ISSN: 0006-4971,1528-0020

DOI: 10.1182/blood.v46.5.705.bloodjournal465705